Computational Sensorics / Communication Engineering (CSE) » Microscopic Focal Stacking

Microscopic Focal Stacking

Author: Stefanie Homberger

Aim of the project: Get 3D info from focused/defocused microscopic images. The task was to capture microscope images using a camera while the object is moved through a plane of focus using a motorized stage. The images contain various focal planes, which can be later merged into a fully focused image through post-processing using algorithms. To achieve automated image capture, I wrote a Python code that controls both the motor and the camera. This code allows the camera to take pictures automatically as the object stage follows a predefined path.

Input: three shallow depth-of-field images from the focal stack of the eye of a grasshopper.
Output: an all-sharp image of the grasshopper eye.


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Aktualisiert um 4:23 PM am 2023-09-06 von ii164652